IRS1 Rabbit Polyclonal Antibody

• Catalog Number: orb10936
• Category: Antibodies
• Description: IRS1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Equine, Human, Porcine, Rabbit, Sheep
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from rat IRS-1 (1101-1200/1242aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-300
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 137 kDa
• Target: IRS1
• UniProt ID: P35570
• RRID: AB_10749870
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: IRS1_HUMAN; Insulin receptor substrate 1; IRS-1; I
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Ning, Chong et al. Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro Mol Nutr Food Res, 61, (2017)

PubMed: 28105758

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRS1 Polyclonal Antibody, Unconjugated (orb10936) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRS1 Polyclonal Antibody, Unconjugated (orb10936) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRS1 Polyclonal Antibody, Unconjugated (orb10936) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.