IRS1 (phospho-Ser616) antibody

• Catalog Number: orb184392
• Category: Antibodies
• Description: IRS1 (phospho-Ser616) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Ser616 (PM(p-S)PG)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, ICC=1:100, Flow-Cyt=1μg/Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 131 kDa
• Target: phospho-IRS1 (Ser616)
• UniProt ID: P35568
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: IRS1 (phospho S616); p-IRS1 (phospho S616); IRS1_H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry of HepG2.Primary Antibody (green line): Rabbit Anti-phospho-IRS1 (Ser616) antibody; Dilution:1μg /10^6 cells.

ICC analysis of A549 cell using phospho-IRS1 (Ser616) polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.

IHC-P analysis of rat uterus using phospho-IRS1 Ser616 Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of Mouse brain using phospho-IRS1 Ser616 Polyclonal Antibody at 1:400 dilution.

Western blot:Lane 1:Mouse Cerebrum;Lane 2:Mouse Cerebellum;Primary:Anti-phospho-IRS1 (Ser616) at 1:1000 dilution.