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| Catalog Number | orb764325 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to IRS-1 (phospho-Ser312) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser312. AA range:279-328 |
| Concentration | 1 mg/ml |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Conjugation | Unconjugated |
| MW | 170 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-IRS1 antibody, anti-Insulin receptor substrat Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot analysis of various cells using Phospho-IRS-1 (S312) Polyclonal Antibody diluted at 1:1000.
Western blot analysis of 293T Hela mouse-spleen lysis using Phospho-IRS-1 (S312) antibody. Antibody was diluted at 1:1000.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRS-1 (Phospho-Ser312) Antibody.
Immunofluorescence analysis of HeLa cells, using IRS-1 (Phospho-Ser312) Antibody. The picture on the right is blocked with the phospho peptide.
Phospho-IRS1 (Ser312) Rabbit pAb [orb2711207]
ELISA, FC, IHC-P
Human, Rat
Polyclonal
Unconjugated
100 μl, 50 μl
