IRF5 antibody

• Catalog Number: orb18401
• Category: Antibodies
• Description: Goat polyclonal antibody to IRF5
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Dilution range: ELISA: 1:128000, WB: 0.3-1 μg/ml
• Conjugation: Unconjugated
• MW: 56.0; 56.0; 57.8
• Target: IRF5
• Entrez: 3663
• Protein Sequence: QGPWPMHPAGMQ
• RRID: AB_10755398
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti IRF5 antibody, anti interferon regulatory fac
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 57kDa band observed in lysates of cell line A549. In transfected HEK293 transiently expressing IRF5 a strong band of approx. 60kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 57.8kDa according to NP_001092099.1. Recommended concentration, 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HEK293 lysate using IRF5 antibody

Western blot analysis of Human Spleen (Lane 1) and Peripheral Blood Lymphocytes (Lane 2) lysates using IRF5 antibody