IRAK4 Antibody

• Catalog Number: orb1255476
• Category: Antibodies
• Description: IRAK4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human IRAK4 (NP_057207.2).
• Concentration: batch dependent
• Dilution range: WB 1:500 - 1:2000IHC 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 55 kDa
• Target: IRAK4
• UniProt ID: Q9NWZ3
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: IRAK-4 Antibody: IPD1, REN64, IRAK-4, NY-REN-64, I
• Note: For research use only
• Application notes: WB 1:500 - 1:2000IHC 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using IRAK4 antibody (orb1255476) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat testis using IRAK4 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using IRAK4 antibody at dilution of 1:100 (40x lens).