IRAK3 Antibody

• Catalog Number: orb1247357
• Category: Antibodies
• Description: IRAK3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-GHSYGSKPMEKR
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 65kDa band observed in Rat Liver lysates (calculated MW of 67.8kDa according to NP_082955.2). An approx 70kDa is observed in Mouse Bone Marrow lysates but this band disappears in lysates from the knock-out mouse. Recommended concentration: 0.05-0.2ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: IRAK3
• UniProt ID: Q8K4B2
• NCBI: NP_082955.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: IRAK3, interleukin-1 receptor-associated kinase 3,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 65kDa band observed in Rat Liver lysates (calculated MW of 67.8kDa according to NP_082955.2). An approx 70kDa is observed in Mouse Bone Marrow lysates but this band disappears in lysates from the knock-out mouse. Recommended concentration: 0.05-0.2ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247357 (0.05 μg/ml) staining of Rat Liver lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected with chemiluminescence.

orb1247357 (0.5 μg/ml) staining of Mouse Bone Marrow (wildtype first lane, KO second lane) lysates (35 μg protein in RIPA buffer). Primary incubation was overnight at 4C Detected with chemiluminescence.