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Catalog Number | orb215979 |
---|---|
Category | Antibodies |
Description | IP3 receptor/ITPR1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human IP3 receptor recombinant protein (Position: A2411-A2758). Human IP3 receptor shares 98% and 97% amino acid (aa) sequences identity with mouse and rat IP3 receptor, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 313929 MW |
UniProt ID | Q14643 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Inositol 1,4,5-trisphosphate receptor type 1;IP3 r Read more... |
Note | For research use only |
Application notes | WB: The detection limit for IP3 receptor is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U87 cells using anti-IP3 receptor antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of IP3 receptor using anti-IP3 receptor antibody.Lane 1:human HeLa cell;2:rat brain tissue;3:mouse brain tissue.
IF analysis of IP3 receptor using anti-IP3 receptor antibody. IP3 receptor was detected in immunocytochemical section of U20S cells.
IHC analysis of IP3 receptor using anti-IP3 receptor antibody.IP3 receptor was detected in paraffin-embedded section of Mouse Brain Tissue.
IHC analysis of IP3 receptor using anti-IP3 receptor antibody.IP3 receptor was detected in paraffin-embedded section of Rat Brain Tissue.
IHC analysis of IP3 receptor using anti-IP3 receptor antibody.IP3 receptor was detected in paraffin-embedded section of Human Lung Cancer Tissue.
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