Interferon gamma Antibody / IFNG

• Catalog Number: orb640107
• Category: Antibodies
• Description: Recognizes a protein of 20-25kDa, identified as human interferon. This MAb is specific to human IFN-g and recognizes both recombinant and native human IFN-gamma. T lymphocytes and NK cells mainly produce IFN-g. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses, including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak anti-viral and anti-proliferative activity, and potentiates the antiviral and anti-tumor effects of IFN-a/b (type I interferon).
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: IFNG/3996R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Purified recombinant human IFNG was used as the immunogen for the recombinant Interferon gamma antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Interferon gamma antibody is available for research use only.
• UniProt ID: P01579
• Storage: Store the recombinant Interferon gamma antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human kidney with recombinant Interferon gamma antibody (clone IFNG/3996R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human kidney with recombinant Interferon gamma antibody (clone IFNG/3996R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.