Integrin Alpha V/Beta 6 antibody

• Catalog Number: orb100289
• Category: Antibodies
• Description: Integrin Alpha V/Beta 6 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Integrin Alpha V + Beta 6
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 18/95/113 kDa
• Target: Integrin Alpha V + Beta 6
• UniProt ID: P06756
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Integrin alpha 5 beta 6; CD51; Integrin alpha-V li
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

L Dirheimer 1, T Pons 2, A François 1 3, L Lamy 1 3, S Cortese 4, F Marchal 1 4, L Bezdetnaya Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680 Cancer Cell Int ., 24, 228 (2024)

PubMed: 38951897

Applications

IHC

Reactivity

Human

Paraffin-embedded human gastric carcinoma fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). Integrin Alpha V/Beta 6 antibody at 1:200 dilution with overnight incubatio

Western blot analysis of Rat Cerebrum Lysate using Integrin Alpha V/Beta 6 antibody.

Western blot analysis of Human Siha Cell Lysate using Integrin Alpha V/Beta 6 antibody.

Western blot analysis of Mouse Kidney Lysate using Integrin Alpha V/Beta 6 antibody.

Flow cytometric analysis of Hela cells using Integrin Alpha V/Beta 6 antibody.