Integrin Alpha V + Beta 6 Rabbit Polyclonal Antibody

• Catalog Number: orb100289
• Category: Antibodies
• Description: Integrin Alpha V + Beta 6 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Integrin Alpha V + Beta 6
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 18/95/113 kDa
• Target: ITGAV
• UniProt ID: P06756
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Integrin alpha 5 beta 6; CD51; Integrin alpha-V li
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

L Dirheimer 1, T Pons 2, A François 1 3, L Lamy 1 3, S Cortese 4, F Marchal 1 4, L Bezdetnaya Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680 Cancer Cell Int ., 24, 228 (2024)

PubMed: 38951897

Applications

IHC

Reactivity

Human

Blank control (blue): Hela (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Integrin Alpha V + Beta 6 antibody (orb100289), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug, Lane 2: MCF-7 (Human) Cell Lysate at 30 ug, Lane 3: Huvec (Human) Cell Lysate at 30 ug, Primary: Anti-CD51 (orb100289) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 125-140 kD, Observed band size: 140 kD.

Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug, Lane 2: U251 (Human) Cell Lysate at 30 ug, Lane 3: Huvec (Human) Cell Lysate at 30 ug, Primary: Anti-Integrin Alpha V + Beta 6 (orb100289) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 125-140 kD, Observed band size: 120 kD.

Tissue/cell: human gastric carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Integrin Alpha V+Beta 6 Polyclonal Antibody, Unconjugated (orb100289) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.