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Integrin Alpha V + Beta 1 Rabbit Polyclonal Antibody

Catalog Number: orb13515

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb13515
CategoryAntibodies
DescriptionIntegrin Alpha V + Beta 1 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, WB
Predicted ReactivityBovine, Canine, Equine, Mouse, Porcine, Sheep
ReactivityHuman, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic Mixed peptides 915-948/1048(human Integrin αV)+ 21-70/798(human Integrin β1)
Concentration1mg/ml
Dilution rangeWB=1:500-2000, Flow-Cyt=1μg /test
Form/AppearanceLiquid
ConjugationUnconjugated
MW114 kDa
TargetITGAV
UniProt IDP06756
RRIDAB_10748950
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesIntegrin alpha 5 beta 1; fibronectin receptor alph
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NoteFor research use only
Expiration Date12 months from date of receipt.

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Hamster
Integrin Alpha V + Beta 1 Rabbit Polyclonal Antibody

Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4°C). Primary Antibody (green line): Rabbit Anti-Integrin Alpha V + Beta 1 antibody (orb13515), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test.

Integrin Alpha V + Beta 1 Rabbit Polyclonal Antibody

Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug, Lane 2: U251 (Human) Cell Lysate at 30 ug, Lane 3: Huvec (Human) Cell Lysate at 30 ug, Lane 4: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 5: U2os (Human) Cell Lysate at 30 ug, Lane 6: MCF-7 (Human) Cell Lysate at 30 ug, Lane 7: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-Integrin Alpha V + Beta 1 (orb13515) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 125 kD, Observed band size: 125 kD.