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Catalog Number | orb13515 |
---|---|
Category | Antibodies |
Description | Integrin Alpha V + Beta 1 Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, WB |
Predicted Reactivity | Bovine, Canine, Equine, Mouse, Porcine, Sheep |
Reactivity | Human, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic Mixed peptides 915-948/1048(human Integrin αV)+ 21-70/798(human Integrin β1) |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, Flow-Cyt=1μg /test |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 114 kDa |
Target | ITGAV |
UniProt ID | P06756 |
RRID | AB_10748950 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | Integrin alpha 5 beta 1; fibronectin receptor alph Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
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Sato, Ryuta et al. Angiopoietin-like protein 2 induces androgen-independent and malignant behavior in human prostate cancer cells Oncol Rep, 33, 58-66 (2015)
Applications
Reactivity
Reichhart, Nadine et al. Rab27a GTPase modulates L-type Ca2+ channel function via interaction with the II-III linker of CaV1.3 subunit Cell Signal, 27, 2231-2240 (2015)
Applications
Reactivity
Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4°C). Primary Antibody (green line): Rabbit Anti-Integrin Alpha V + Beta 1 antibody (orb13515), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test.
Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug, Lane 2: U251 (Human) Cell Lysate at 30 ug, Lane 3: Huvec (Human) Cell Lysate at 30 ug, Lane 4: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 5: U2os (Human) Cell Lysate at 30 ug, Lane 6: MCF-7 (Human) Cell Lysate at 30 ug, Lane 7: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-Integrin Alpha V + Beta 1 (orb13515) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 125 kD, Observed band size: 125 kD.
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Human, Rat | |
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