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Catalog Number | orb315156 |
---|---|
Category | Antibodies |
Description | Integrin alpha 4/ITGA4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC, IHC-Fr, WB |
Reactivity | Human, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin alpha 4 (999-1028aa MWKAGFFKRQYKSILQEENRRDSWSYINS K), different from the related mouse sequence by one amino acid. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 114900 MW |
UniProt ID | P13612 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Integrin alpha-4;CD49 antigen-like family member D Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of THP-1 cells using anti-Integrin alpha 4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of Integrin alpha 4 using anti-Integrin alpha 4 antibody.Lane 1:Rat Liver Tissue;2:Rat Kidney Tissue;3:JURKAT Cell;4:22RV1 Cell;5:HEPG2 Cell;6:SMMC Cell.
IHC analysis of Integrin alpha 4 using anti-Integrin alpha 4 antibody. Integrin alpha 4 was detected in a paraffin-embedded section of human mammary cancer tissue.
IHC analysis of Integrin alpha 4 using anti-Integrin alpha 4 antibody.Integrin alpha 4 was detected in frozen section of human placenta tissues.
ELISA | |
Bovine, Canine, Equine, Porcine, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
FC, WB | |
Bovine, Equine, Mouse, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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