Insulin R (phospho-Thr1375) antibody

• Catalog Number: orb768782
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Insulin R (phospho-Thr1375)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human IR around the phosphorylation site of Thr1375. AA range:1331-1380
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 130
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-INSR antibody, anti-Insulin receptor antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IR (Phospho-Thr1375) Antibody.

Western blot analysis of lysates from HepG2 cells, using IR (Phospho-Thr1375) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).