Insulin Antibody

• Catalog Number: orb606442
• Category: Antibodies
• Description: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. [UniProt]
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: IRDN/1980R
• Tested applications: IHC-P
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG, kappa
• Immunogen: Purified pig insulin conjugated to KLH was used as the immunogen for the recombinant INS antibody.
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant INS antibody is available for research use only.
• UniProt ID: P01308
• Storage: Store the recombinant INS antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant INS antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human pancreas with recombinant INS antibody (clone IRDN/1980R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant INS antibody (clone IRDN/1980R) as confirmation of integrity and purity.