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Catalog Number | orb750685 |
---|---|
Category | Antibodies |
Description | IL8 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, IP, WB |
Reactivity | Human |
Isotype | Antiserum |
Immunogen | The whole rabbit serum was prepared by repeated immunizations with recombinant human IL-8 produced in E.coli. |
Concentration | 75 mg/mL |
Dilution range | ELISA: 1:1,000 - 1:5,000, IHC: 1:400 - 1:800, IP: 1:400 - 1:800, WB: 1:500 - 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-IL 8 antiserum has been heated to 56°C for 30 minutes. The antiserum detects recombinant and native IL-8 present in body fluids and cell supernatants in ELISA. Although likely to be functional, this antiserum has not been evaluated in other assays, including neutralization and immunohistochemistry. |
Conjugation | Unconjugated |
UniProt ID | P10145 |
NCBI | P10145.1 |
Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | None |
Alternative names | Anti-CXCL 8, CXCL8 antibody, 310C antibody, AMCF 1 Read more... |
Note | For research use only |
Application notes | IL 8 antibody has been tested for use in ELISA and western blot. It recognizes IL-8 found in cells, supernatants and other body fluids. Reactivity in other immunoassays is unknown. |
Expiration Date | 12 months from date of receipt. |
Western Blot of Rabbit anti-Human IL-8 Antibody. Lane 1: Recombinant Human IL-8. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human IL-8 Antibody 1:1000 overnight at 4°C. Secondary antibody: HRP Goat-a-rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 11 kDa, 11 kDa for Human IL-8.
FC, ICC, IHC-P, WB | |
Bovine, Equine, Gallus, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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