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Catalog Number | orb750684 |
---|---|
Category | Antibodies |
Description | IL8 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, IP, WB |
Reactivity | Human |
Isotype | Antiserum |
Immunogen | The whole rabbit serum was prepared by repeated immunizations with recombinant human IL-8 produced in E.coli. |
Concentration | 75 mg/ml |
Dilution range | ELISA: 1:1,000 - 1:5,000, IHC: 1:400 - 1:800, IP: 1:400 - 1:800, WB: 1:500 - 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-IL 8 antiserum has been heated to 56°C for 30 minutes. The antiserum detects recombinant and native IL-8 present in body fluids and cell supernatants in ELISA. Although likely to be functional, this antiserum has not been evaluated in other assays, including neutralization and immunohistochemistry. |
Conjugation | Unconjugated |
UniProt ID | P10145 |
NCBI | P10145.1 |
Storage | Store anti-IL8 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | None |
Alternative names | Anti-CXCL 8, CXCL8 antibody, 310C antibody, AMCF 1 Read more... |
Note | For research use only |
Application notes | IL 8 antibody has been tested for use in ELISA and western blot. It recognizes IL-8 found in cells, supernatants and other body fluids. Reactivity in other immunoassays is unknown. |
Expiration Date | 12 months from date of receipt. |
Western Blot of Rabbit anti-Human IL-8 Antibody. Lane 1: Recombinant Human IL-8. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human IL-8 Antibody 1:1000 overnight at 4°C. Secondary antibody: HRP Goat-a-rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 11 kDa, 11 kDa for Human IL-8.
FC, ICC, IHC-P, WB | |
Bovine, Equine, Gallus, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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