IL8 antibody

• Catalog Number: orb750683
• Category: Antibodies
• Description: IL8 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Human
• Isotype: Antiserum
• Immunogen: The whole rabbit serum was prepared by repeated immunizations with recombinant human IL-8 produced in E.coli.
• Concentration: 75 mg/mL
• Dilution range: ELISA: 1:1,000 - 1:5,000, IHC: 1:400 - 1:800, IP: 1:400 - 1:800, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-IL 8 antiserum has been heated to 56°C for 30 minutes. The antiserum detects recombinant and native IL-8 present in body fluids and cell supernatants in ELISA. Although likely to be functional, this antiserum has not been evaluated in other assays, including neutralization and immunohistochemistry.
• Conjugation: Unconjugated
• UniProt ID: P10145
• NCBI: P10145.1
• Storage: Store anti-IL8 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: None
• Alternative names: Anti-CXCL 8, CXCL8 antibody, 310C antibody, AMCF 1
• Note: For research use only
• Application notes: IL 8 antibody has been tested for use in ELISA and western blot. It recognizes IL-8 found in cells, supernatants and other body fluids. Reactivity in other immunoassays is unknown.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Human IL-8 Antibody. Lane 1: Recombinant Human IL-8. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human IL-8 Antibody 1:1000 overnight at 4°C. Secondary antibody: HRP Goat-a-rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 11 kDa, 11 kDa for Human IL-8.