IL6 antibody (Peroxidase)

• Catalog Number: orb345157
• Category: Antibodies
• Description: IL6 antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-6 produced in E.coli.
• Concentration: 1.1 mg/ml
• Dilution range: ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000
• Form/Appearance: Lyophilized
• Purity: This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human Il-6 in cell supernatants and certain body fluids. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. A control of similarly diluted normal rabbit IgG is recommended. This product has minimal reactivity with mouse IL-6.
• Conjugation: HRP
• UniProt ID: P05231
• NCBI: NP_000591.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit Anti-IL-6 peroxidase conjugated antibody, r
• Note: For research use only
• Application notes: This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western Blot of Peroxidase conjugated Rabbit anti-Human IL-6 antibody. Lane 1: Human IL-6. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase Human IL-6 secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 20 kDa for Human IL-6. Other band(s): none.

Western Blot of Rabbit Anti-IL-6 Antibody Peroxidase Conjugated. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: IL-6/MOLT-4 lysate (p/n orb348696) [0.01/10 µg]. Lane 3: IL-6/MOLT-4 lysate (p/n orb348696) [0.005/10 µg]. Lane 4: IL-6/MOLT-4 lysate (p/n orb348696) [0.002/10 µg]. Primary Antibody: Anti-IL-6 HRP conjugated at 1.0 µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP mx10 (p/n orb347654) at 1:70000 for 30 min at RT. Blocking: BlockOut Buffer (p/n orb348644) at RT for 1hr. Predicted/Observed MW: ~20.6 kDa. Exposure: 0.5sec.