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Catalog Number | orb345166 |
---|---|
Category | Antibodies |
Description | IL4 antibody (Peroxidase) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-4 protein. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: 1:500;1:2,500, WB: 1:1,000-1:5,000 |
Form/Appearance | Lyophilized |
Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-4 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended. |
Conjugation | HRP |
UniProt ID | P05112 |
NCBI | NP_000580.1 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | rabbit anti-IL-4 peroxidase conjugated antibody, r Read more... |
Note | For research use only |
Application notes | This purified antibody has been tested in western blotting and suitable for ELISA. By western blot a band approximately 15 kDa in size corresponding to human IL-4 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. |
Expiration Date | 12 months from date of receipt. |
Western Blot of Rabbit Anti-IL4 Antibody Peroxidase Conjugated. Lane 1: Human IL-4. Load: 50 ng per lane. Secondary antibody: IL-4 Antibody Peroxidase Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 30 min RT. Predicted/Observed size: 18 kDa, 18 kDa.
Hamster | |
15.63-1000 pg/mL | |
5.7 pg/mL |
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