IL4 antibody (Biotin)

• Catalog Number: orb345181
• Category: Antibodies
• Description: IL4 antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-4 protein.
• Concentration: 0.80 mg/ml
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
• Form/Appearance: Lyophilized
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-4 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
• Conjugation: Biotin
• UniProt ID: P05112
• NCBI: NP_000580.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-IL-4 biotin conjugated antibody, rabbi
• Note: For research use only
• Application notes: This purified antibody has been tested in western blotting and suitable for ELISA. By western blot a band approximately 15 kDa in size corresponding to human IL-4 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western Blot of Biotin Conjugated Rabbit anti-IL-4 Antibody. Lane 1: Human IL-4. Lane 2: none. Load: 50 ng per lane. Primary antibody: IL-4 Antibody Biotin Conjugated at 1:1000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-4. Other band(s): none.