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Catalog Number | orb1671458 |
---|---|
Category | Antibodies |
Description | Il4 Antibody (Biotin) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Mouse |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure recombinant Murine IL-4. Anti-Murine IL-4 specific antibody was purified by affinity chromatography and then biotinylated. |
Concentration | batch dependent |
Form/Appearance | Lyophilized |
Conjugation | Biotin |
Target | Il4 |
UniProt ID | P07750 |
NCBI | P07750 |
Storage | Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | BCGF, BCDF, B cell stimulating factor (BSF-1) Read more... |
Note | For research use only |
Application notes | Sandwich ELISA: To detect Murine IL-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Murine IL-4 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine IL-4. Western Blot To detect Murine IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Murine IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
To detect Murine IL-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IL-4 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine IL-4.
To detect Murine IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Murine IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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