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Catalog Number | orb1272663 |
---|---|
Category | Antibodies |
Description | IL31 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-31. Human IL-31 specific antibody was purified by affinity chromatography employing immobilized hIL-31 matrix. |
Concentration | batch dependent |
Dilution range | ELISA:Indirect:To detect IL-31 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant IL-31.SandwichTo detect IL-31 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human IL-31 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant IL-31. Western Blot:To detect IL-31 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant IL-31 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | IL31 |
UniProt ID | Q6EBC2 |
NCBI | Q6EBC2 |
Storage | The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | IL-31Interleukin-31IL-31 Read more... |
Note | For research use only |
Application notes | ELISA:Indirect:To detect IL-31 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant IL-31.SandwichTo detect IL-31 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human IL-31 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant IL-31. Western Blot:To detect IL-31 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant IL-31 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
To detect hIL-31 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-31 (orb1272721) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-31.
To detect hIL-31 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-31 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hIL-31 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-31 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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