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Catalog Number | orb1238893 |
---|---|
Category | Antibodies |
Description | IL27 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, WB |
Predicted Reactivity | Rat |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Anti-IL-27 antibody (orb1238893) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human IL-27. The immunogen is located within the first 50 amino acids of IL-27. |
Concentration | 1 mg/mL |
Dilution range | WB: 0.5 - 4 μg/mL; ICC: 10 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in mouse samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 28 kDa Observed: 28 kDa |
Target | IL27 |
UniProt ID | Q8NEV9 |
NCBI | NP_663634 |
Storage | IL-27 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | IL-27 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | IL-27 Antibody: p28, IL30, IL-27, IL27A, IL-27A, I Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot Validation in Mouse A20 Cells. Loading: 15 µg of lysates per lane. Antibodies: IL-27 orb1238893, (A: 1 µg/mL, B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: IL-27 orb1238893 (4 µg/mL), IL-27 orb1239663 (4 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17 (IL-17A+IL-17B) protein per lane. Antibodies: IL-27 orb1238893, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL, Lane 3: 2 µg/mL.
Immunocytochemistry Validation of IL-27 in Mouse EL4 Cells. Immunocytochemical analysis of Mouse EL4 cells using anti-IL-27 antibody (orb1238893) at 10 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
ELISA, WB | |
Porcine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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