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Catalog Number | orb1238905 |
---|---|
Category | Antibodies |
Description | IL21R Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | IL-21 receptor antibody was raised against a synthetic peptide corresponding to amino acids 97 to 111 of human IL-21 receptor precursor.The immunogen is located within amino acids 80 - 130 of IL-21 Receptor. |
Concentration | 1 mg/ml |
Dilution range | IL-21 receptor antibody can be used for detection of IL-21 receptor by Western blot at 1-2 μg/mL. An approximately 60 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 60 kDa Observed: 62 kDa |
Target | IL21R |
UniProt ID | Q9HBE5 |
NCBI | NP_068570 |
Storage | IL-21 Receptor antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | IL-21 Receptor Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | IL-21 Receptor Antibody: NILR, CD360, NILR, UNQ312 Read more... |
Note | For research use only |
Application notes | IL-21 receptor antibody can be used for detection of IL-21 receptor by Western blot at 1-2 μg/mL. An approximately 60 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of IL-21 receptor expression in human HepG2 cell lysate with IL-21 receptor antibody at (A) 1 and (B) 2 µg/ml.
Immunohistochemistry of IL-21 receptor in rat lung with IL-21 receptor antibody at 10 µg/mL.
Immunofluorescence of IL-21 Receptor in Rat Lung cells with IL-21 Receptor antibody at 20 µg/mL.
Immunofluorescence of IL21R in A431 cells with IL 21 Receptor antibody at 20 µg/mL. Green: IL-21 Receptor Antibody (orb1238905) Blue: DAPI staining.
ELISA, IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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