IL1B Antibody (Biotin)

• Catalog Number: orb1671473
• Category: Antibodies
• Description: IL1B Antibody (Biotin)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of Goats pre-immunized with highly pure (>98%) recombinant hIL-1 beta. Anti-HumanIL-1beta specific antibody was purified by affinity chromatography and then biotinylated.
• Concentration: batch dependent
• Form/Appearance: Lyophilized
• Conjugation: Biotin
• Target: IL1B
• UniProt ID: P01584
• NCBI: P01584
• Storage: Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: Catabolin, Lymphocyte-activating factor (LAF), End
• Note: For research use only
• Application notes: Sandwich ELISA: To detect hIL-1 beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human IL-1 beta as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-1 beta. Western Blot To detect hIL-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1 beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect hIL-1 beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-1 beta as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-1 beta.

To detect hIL-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.