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Catalog Number | orb1471918 |
---|---|
Category | Proteins |
Description | IL-17E Human Recombinant produced in HEK293 cells is a single, non-glycosylated polypeptide chain containing 154 amino acids (33-177a.a) and having a molecular mass of 17.8kDa.IL-17E is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques. |
Form/Appearance | Sterile Filtered colorless solution. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Protein Sequence | DGSYSHWPSC CPSKGQDTSE ELLRWSTVPV PPLEPARPNR HPESCRASED GPLNSRAISP WRYELDRDLN RLPQDLYHAR CLCPHCVSLQ TGSHMDPRGN SELLYHNQTV FYRRPCHGEK GTHKGYCLER RLYRVSLACV CVRPRVMGHH HHHH |
Source | HEK293 |
Biological Activity | Measured by its binding ability in a functional ELISA with Human IL-17 RB. |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
Buffer/Preservatives | IL-17E protein (0.5mg/ml) contains Phosphate-Buffered Saline (pH 7.4) and 20% glycerol. |
Alternative names | interleukin-25 isoform 1, interleukin 25, IL17E, I Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 42.9 kDa after removal of the signal peptide. The apparent molecular mass of IL25-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
SDS-PAGE: 100%; SEC-HPLC: 97% | |
20-26 KDa (reducing condition) |
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | |
Escherichia Coli |