IL13 antibody

• Catalog Number: orb344653
• Category: Antibodies
• Description: IL13 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Porcine, Sheep
• Isotype: IgG
• Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant protein raised in yeast, corresponding to mature swine IL-13 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000, WB: 2 µg/mL
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for swine IL-13 protein. A BLAST analysis was used to suggest reactivity with IL-13 from swine sources based on 100% homology with the immunizing sequence. The following homologies may indicate chances of cross-reactivity when amino acid similarity and not just exact sequence is accounted: 89-90% homology to IL-13 from sheep, dolphin, beluga, and cattle, 83-88% to human and certain monkey IL-13, 80-82% to dusky titi, macaque, baboon, mangabey, dog, llama, camel, and galago, 75-78% to horse, and 67-72% to rat, mouse, and gerbil. Cross-reactivity with IL-13 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: B3GDZ5
• NCBI: 189176151
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Interleukin-13 antibody, rabbit anti-I
• Note: For research use only
• Application notes: This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13.2 kDa in size corresponding to swine IL-13 protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's protein-A purified anti-swine IL-13 antibody shows detection of recombinant swine IL-13 at 13.2 kDa raised in yeast. Multiple bands are expected of the glycosylated protein. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (diluted 1:10), and probed with Biorbyt's Anti-swine IL-13. After washing, membrane was probed with Dylight ™ 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.