IL13 antibody

• Catalog Number: orb344652
• Category: Antibodies
• Description: IL13 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Porcine, Sheep
• Isotype: IgG
• Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant protein raised in yeast, corresponding to mature swine IL-13 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:11,000, WB: 1.5 to 2 µg/ml
• Form/Appearance: Lyophilized
• Purity: This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for swine IL-13 protein. A BLAST analysis was used to suggest reactivity with IL-13 from swine sources based on 100% homology with the immunizing sequence. The following homologies may indicate chances of cross-reactivity when amino acid similarity and not just exact sequence is accounted: 89-90% homology to IL-13 from sheep, dolphin, beluga, and bovine, 83-88% to human and certain monkey IL-13, 80-82% to dusky titi, macaque, baboon, mangabey, dog, llama, camel, and galago, 75-78% to horse, and 67-72% to rat, mouse, and gerbil. Cross-reactivity with IL-13 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: B3GDZ5
• NCBI: 189176151
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Interleukin-13 antibody, rabbit anti-I
• Note: For research use only
• Application notes: IL-13 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13.2 kDa in size corresponding to swine IL-13 protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's protein-A purified anti-swine IL-13 antibody shows detection of recombinant swine IL-13 at 13.2 kDa raised in yeast. Multiple bands are expected of the glycosylated protein. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (diluted 1:10), and probed with Biorbyt's Anti-swine IL-13. After washing, membrane was probed with Dylight ™ 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.