IL12RB1 Antibody

• Catalog Number: orb1258406
• Category: Antibodies
• Description: IL12RB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-305 of human IL12RB1 (NP_714912.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:10 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 73kDa
• Target: IL12RB1
• UniProt ID: P42701
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: IL12RB1, MGC34454, interleukin-12 receptor beta-1
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:10 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using IL12RB1 antibody (orb1258406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Immunofluorescence analysis of A549 cells using IL12RB1 antibody (orb1258406). Blue: DAPI for nuclear staining.