IL12B Antibody

• Catalog Number: orb1270811
• Category: Antibodies
• Description: IL12B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This IL12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-290 amino acids from the C-terminal region of human IL12B.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 37 kDa
• Target: IL12B
• UniProt ID: P29460
• NCBI: P29460
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Interleukin-12 subunit beta, IL-12B, Cytotoxic lym
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

IL12B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in CEM, MDA-MB453 cell line lysates (35 ug/lane).