IL11 Antibody

• Catalog Number: orb1272265
• Category: Antibodies
• Description: IL11 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 27B1047.1
• Tested applications: ELISA, NeA, WB
• Reactivity: Human
• Immunogen: The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human IL-11 as the immunizing antigen.
• Concentration: batch dependent
• Dilution range: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of human IL-11 (5.0 ng/mL), a concentration of 2.4 - 3.6 μg/mL of this antibody is required. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 8-9 μg/mL of this antibody will detect at least 0.2 ng/well of recombinant human IL-11 when used with our biotinylated antigen affinity purified anti-human IL-11(XP-5164Bt) as the detection antibody at a concentration of at least 2 μg/mL.Western blot:To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: IL11
• UniProt ID: P20809
• NCBI: P20809
• Storage: IL-11 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
• Alternative names: Interleukin 11Interleukin-11, Adipogenesis inhibit
• Note: For research use only
• Application notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of human IL-11 (5.0 ng/mL), a concentration of 2.4 - 3.6 μg/mL of this antibody is required. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 8-9 μg/mL of this antibody will detect at least 0.2 ng/well of recombinant human IL-11 when used with our biotinylated antigen affinity purified anti-human IL-11(XP-5164Bt) as the detection antibody at a concentration of at least 2 μg/mL.Western blot:To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To a sandwich ELISA (assuming 100ul/well), a concentration of 8.0-9.0 ug/ml of this antibody will detect recombinant human IL-11 when used with antigen affinity purified anti-human IL-11 (orb1272490) as the detection antibody at a concentration of approximately 2.0-4.0 ug/ml.

To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.

To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.

This antibody stained human brain stroke (including control cortex and stroke core areas) tissue. The primary antibody was incubated at 2.5 ug/ml overnight at 4°C. This was followed by a fluorophore conjugated secondary antibody. Optimal concentrations and conditions may vary.