IL-8 antibody

• Catalog Number: orb345090
• Category: Antibodies
• Description: IL-8 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Feline
• Isotype: IgG
• Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to 79 amino acids of mature cat IL-8 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:50,000, WB: 1:500-1:2,000
• Form/Appearance: Lyophilized
• Purity: This product was protein A purified from monospecific antiserum by chromatography. This antibody is specific for cat IL-8 protein. A BLAST analysis was used to suggest cross-reactivity with IL-8 from cat sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-8 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q9XSX5
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: IL8, rabbit anti-IL-8 antibody, rabbit anti-Interl
• Note: For research use only
• Application notes: This protein A purified CXCL8 antibody has been tested by Western blotting and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 8.9 kDa in size corresponding to cat IL-8 by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's protein-A purified anti-Cat IL-8 antibody shows detection of recombinant Cat IL-8 at 8.9 kDa, raised in yeast. Primary antibody was diluted to 1 µg/ml. 3% BSA from Biorbyt's Bovine Serum Albumin Solution was used for blocking. Secondary antibody (Goat anti-Rabbit IgG IRDye® 800) was used at 1:20000.