IL-7 Receptor Alpha Chain (phospho-Y449) antibody

• Catalog Number: orb345608
• Category: Antibodies
• Description: IL-7 Receptor Alpha Chain (phospho-Y449) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.
• Concentration: 0.72 mg/mL
• Dilution range: ELISA: 1:5,000-1:50,000, IHC: User Optimized, WB: 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against non-phospho-peptide. This antibody is specific for mouse IL-7 protein phosphorylated at Y449. A BLAST analysis was used to suggest cross-reactivity with IL-7 from human, mouse and rat sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-7 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P16872
• NCBI: 45269152
• Storage: Store IL-7 Receptor Alpha Chain phospho Y449 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-IL-7 Receptor alpha pY449 antibody, ra
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 55 kDa in size corresponding to phosphorylated IL-7 by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse IL-7 pY449 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
• Expiration Date: 12 months from date of receipt.

Western Blot and immunoprecipitation of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody. Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/mL). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. Primary antibody: IL7pY449 antibody at 1:10000 for overnight at 4°C. Secondary antibody: HRP-conjugated goat anti-rabbit antibody at 1:10000 for 45 min at RT and ECL detection. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.

Western Blot of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody. Load: thymocyte D1 cells treated with or without IL-7 (50 ng/mL) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.