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Catalog Number | orb1231156 |
---|---|
Category | Proteins |
Description | IL-7 R alpha / CD127 Recombinant Protein |
Tested applications | ELISA, WB |
Tag | Mouse IgG Fc Tag |
Form/Appearance | Lyophilized |
Purity | > 90% as determined by SDS-PAGE. |
MW | 52.0 kDa |
Protein Sequence | Glu 21 - Gly 236 |
Source | HEK293 cells |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
Alternative names | IL7Ra,CD127 Read more... |
Note | For research use only |
Application notes | This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 52.0 kDa. The protein migrates as 65-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Human IL-7 R alpha, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized ActiveMax Human IL-7, Tag Free at 2 ug/ml (100 uL/well) can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag with a linear range of 4-31 ng/mL.
Biotinylated Human TSLP (R127A, R130A), His, Avitag immobilized at 2 ug/ml (100 uL/well) via precoated 5 ug/ml (100 uL/well) of Human TSLP R, Fc Tag, can bind can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag