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Catalog Number | orb1231525 |
---|---|
Category | Proteins |
Description | IL-6 Recombinant Protein |
Tested applications | ELISA, WB |
Tag | His Tag |
Form/Appearance | Lyophilized |
Purity | > 90% as determined by SDS-PAGE. |
MW | 23.6 kDa |
Target | Il6 |
UniProt ID | P08505 |
Protein Sequence | Phe 25 - Thr 211 |
Source | HEK293 cells |
NCBI | NP_112445.1 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.4 |
Alternative names | IL6,Interleukin-6,BSF2,HSF,IFNB2 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 23.6 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Mouse IL-6, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Mouse Human IL-6, His Tag at 5 ug/ml (100 uL/well) can bind Biotinylated Mouse IL-6 R alpha, His, Avitag with a linear range of 20-156 ng/mL.
Greater than 95% as determined by SDS-PAGE. | |
20.9 kDa | |
E.coli |
> 96% as determined by SDS-PAGE and HPLC. | |
20.7 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
21.1 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
21.7 kDa | |
E.Coli |