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Catalog Number | orb1955843 |
---|---|
Category | Proteins |
Description | IL-35 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 102 kDa and the accession number is P29459-1. |
Tag | Tag Free |
Purity | 98.00% |
MW | 102 kDa (predicted); 60 & 63 kDa (reducing conditions) |
UniProt ID | P29459 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | IL-35 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 102 kDa and the accession number is P29459-1. |
Expression Region | A DNA sequence encoding the p35 subunit of human IL12, termed as IL12A (P29459-1) (Met1-Ser219) was fused with a C-terminal Fc region of human IgG1 tag followed by a His tag, constructed the plasmid 1; A DNA sequence encoding the human IL27B, (NP_005746.2) (Met1-Lys229) was fused with a C-terminal Fc region of human IgG1 tag followed by a FLAG tag, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. Predicted N terminal: Arg 23 & Arg 21 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
98.00% | |
102.1 kDa (predicted); 66 kDa (reducing conditions) |
96.70% | |
48.3 kDa (predicted) |