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Catalog Number | orb1954968 |
---|---|
Category | Proteins |
Description | IL-35 Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 48.3 kDa and the accession number is P29459-1. |
Tag | C-Flag, C-His |
Purity | 96.70% |
MW | 48.3 kDa (predicted) |
UniProt ID | P29459 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | IL-35 Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 48.3 kDa and the accession number is P29459-1. |
Expression Region | A DNA sequence encoding the human IL27B (NP_005746.2)(Met1-Lys229) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human IL12A (P29459-1)(Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human IL27B&IL12A heterodimer was purified. Predicted N terminal: Arg 21 & Arg 23 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |