IL-22 antibody

• Catalog Number: orb345156
• Category: Antibodies
• Description: IL-22 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-IL-22 antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human IL-22 protein from E. coli.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:50,000, WB: 1:1000-1:2000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-IL-22 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-22 protein. A BLAST analysis was used to suggest cross-reactivity with IL-22 from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-22 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q9GZX6
• NCBI: NP_065386.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-IL-22 antibody, rabbit anti-interleuki
• Note: For research use only
• Application notes: IL-22 purified antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 20 kDa in size corresponding to the human IL-22 protein by western blotting in appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-IL-22 antibody. Lane 1: Human IL-22 Recombinant Protein. Load: 50 ng per lane. Primary antibody: IL-22 antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 14 kDa, 14 kDa for Human IL-22. Other band(s): None.