IL-2 R alpha / CD25 Recombinant Protein

• Catalog Number: orb1231318
• Category: Proteins
• Description: IL-2 R alpha / CD25 Recombinant Protein
• Tested applications: ELISA, WB
• Tag: His Tag
• Dilution range: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.6 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Form/Appearance: Lyophilized
• Purity: > 90% as determined by SDS-PAGE.
• MW: 26.6 kDa
• Target: Il2ra
• UniProt ID: P01590
• Protein Sequence: Glu 22 - Lys 236
• Source: HEK293 cells
• NCBI: NP_032393.3
• Storage: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4
• Alternative names: IL2RA,CD25,p55,IL2-RA,IL-2-RA
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.6 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Mouse IL-2 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Immobilized Biotinylated Human IL-2, Fc Tag, Avi Tag at 5 ug/ml (100 uL/well) on Streptavidin precoated (0.5 ug/well) plate, can bind Mouse IL-2 R alpha, His Tag with a linear range of 5-40 ng/mL.

Immobilized Mouse IL-2 R alpha, His Tag at 5 ug/ml (100 uL/well) can bind Biotinylated Human IL-2, Fc Tag, Avi Tag with a linear range of 0.6-10 ng/mL.