IL-2 antibody (Peroxidase)

• Catalog Number: orb345168
• Category: Antibodies
• Description: IL-2 antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-2 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000
• Form/Appearance: Lyophilized
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-2 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
• Conjugation: HRP
• UniProt ID: P60568
• NCBI: NP_000577.2
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-IL-2 peroxidase conjugated Antibody, r
• Note: For research use only
• Application notes: This purified antibody has been tested for use in western blotting. By western blot a band approximately 15 kDa in size corresponding to human IL-2 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-IL-2 Antibody Peroxidase Conjugated. Lane 1: 2 ug Human IL-2 Recombinant Protein. Lane 2: 1 ug Hu IL-2 Recombinant Protein. Lane 3: 500 ng Hu IL-2 Recombinant Protein. Lane 4: 250 ng Hu IL-2 Recombinant Protein. Secondary antibody: Peroxidase Conjugated IL-2 Antibody at 1:1000 for 60 min at RT. Block: TBST + 5% BLOTTO overnight. Predicted/Observed size: 16 kDa, 16 kDa. Other bands observed: 30 kDa dimeric form of IL-2.

Western Blot of Rabbit anti-IL-2 Antibody Peroxidase Conjugated. Lane 1: 250 ng glycosylated IL-2. Lane 2: 250 ng non-glycosylated IL-2. Secondary antibody: Peroxidase Conjugated IL-2 Antibody at 1:1000 for 60 min at RT. Block: TBST + 5% BLOTTO overnight. Predicted/Observed size: 16 kDa, 16 kDa. Other bands observed: 30 kDa dimeric form of IL-2.

Western Blot of Rabbit anti-IL-2 Antibody Peroxidase Conjugated. Lane 1: 250 ng glycosylated IL-2. Lane 2: 250 ng non-glycosylated IL-2. Secondary antibody: Peroxidase Conjugated IL-2 Antibody at 1:10000 for 60 min at RT. Lane 3: 250 ng glycosylated IL-2. Lane 4: 250 ng non-glycosylated IL-2. Secondary antibody: Peroxidase Conjugated IL-2 Antibody at 1:20000 for 60 min at RT. Block: TBST + 5% BLOTTO overnight. Predicted/Observed size: 16 kDa, 16 kDa. Other bands observed: 30 kDa dimeric form of IL-2.

Western Blot of Rabbit anti-IL-2 Antibody Peroxidase Conjugated. Lane 1: Human IL-2 Recombinant Protein. Load: 50 ng per lane. Secondary antibody: Peroxidase Conjugated IL-2 Antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 16 kDa, 16 kDa.