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IL-17F Biotin Conjugated Antibody
Catalog Number: orb345116
| Catalog Number | orb345116 |
|---|---|
| Category | Antibodies |
| Description | Human IL-17F antibody (Biotin) |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | 4H1629.1 |
| Tested applications | ELISA, IHC, WB |
| Reactivity | Human |
| Isotype | IgG1 |
| Immunogen | Anti-IL-17F (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17F produced in E.coli followed by hybridoma development. |
| Concentration | 1.0 mg/mL |
| Dilution range | ELISA: 1:10,000-1:50,000, IHC: 5µg/mL, WB: 0.5 mg/mL |
| Form/Appearance | Lyophilized |
| Purity | This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17F protein. A BLAST analysis was used to suggest cross-reactivity with IL-17F from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17F from other sources has not been determined. |
| Conjugation | Biotin |
| UniProt ID | Q96PD4 |
| NCBI | NP_443104.1 |
| Storage | Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | mouse anti-IL-17F biotin conjugated antibody, mous Read more... |
| Note | For research use only |
| Application notes | This purified antibody has been tested for use in IHC and Western Blot. Specific conditions for reactivity should be optimized by the end user. |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemistry of Mouse Anti-IL-17F antibody. Tissue: human colon tissue. Fixation: formalin-fixed, paraffin-embedded. Primary antibody: isotype control (top left), Mouse Anti-IL-17F antibody (right and bottom left) at 5 ug/ml.
Western Blot of Mouse Anti-IL-17F antibody. Lane 1: human full length recombinant IL-17F protein. Lane 2: mouse full length recombinant IL-17F protein. Lane 3: rat full length recombinant IL-17F protein. Load: 20 ng/lane. Primary antibody: Anti-IL-17F antibody (orb345104) at 0.5 ug/ml for overnight at 4°C.
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