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Catalog Number | orb345120 |
---|---|
Category | Antibodies |
Description | IL-17E antibody (Biotin) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 11K8B1 |
Tested applications | ELISA, FC, WB |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | Anti-IL-17E (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17E produced in E.coli followed by hybridoma development. |
Antibody Type | Primary Antibody |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:10,000 - 1:50,000, FC: 1µg/mL, WB: 0.5µg/mL |
Form/Appearance | Lyophilized |
Purity | This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17E protein. A BLAST analysis was used to suggest cross-reactivity with IL-17E from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17E from other sources has not been determined. |
Conjugation | Biotin |
UniProt ID | Q9H293 |
NCBI | NP_073626.1 |
Storage | Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | mouse Anti-IL-17E Biotin Conjugated Antibody, mous Read more... |
Note | For research use only |
Application notes | This purified antibody has been tested for use in Flow Cytometry and western blotting. Specific conditions for reactivity should be optimized by the end user. |
Expiration Date | 12 months from date of receipt. |
Anti-IL-17E Antibody - Flow Cytometry Cells: PC3 cells Primary antibody: 1 ug of IL-17E (red), control (green) Secondary antibody: anti-mouse IgG PE conjugated secondary antibody.
Anti-IL-17E Biotin Conjugated Antibody - Western Blot. Western Blot of Mouse anti-IL-17E antibody. Load: full length recombinant protein. Primary Antibody: anti-IL-17E antibody (orb345112) at 0.5 ug/ml.