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Catalog Number | orb345117 |
---|---|
Category | Antibodies |
Description | IL-17A antibody (Biotin) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 4H1524.1 |
Tested applications | WB |
Reactivity | Human |
Isotype | IgG2b |
Immunogen | Anti-IL-17A (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17A produced in E.coli followed by hybridoma development. |
Antibody Type | Primary Antibody |
Concentration | 1.0 mg/mL |
Dilution range | WB: 1µg/mL |
Form/Appearance | Lyophilized |
Purity | This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17A protein. A BLAST analysis was used to suggest cross-reactivity with IL-17A from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17A from other sources has not been determined. |
Conjugation | Biotin |
UniProt ID | Q16552 |
NCBI | NP_002181.1 |
Storage | Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | mouse anti-Interleukin-17A biotin conjugated antib Read more... |
Note | For research use only |
Application notes | This purified antibody has been tested by Western Blot and suitable for use in FLOW. Specific conditions for reactivity should be optimized by the end user. |
Expiration Date | 12 months from date of receipt. |
Western Blot of Mouse Anti-IL-17A antibody. Lane 1: human full length recombinant IL-17A protein. Lane 2: mouse full length recombinant IL-17A protein. Lane 3: rat full length recombinant IL-17A protein. Load: 20 ng/lane. Primary antibody: Anti-IL-17A antibody (orb345106) at 1 ug/ml for overnight at 4°C.