IL-17A antibody

• Catalog Number: orb345248
• Category: Antibodies
• Description: IL-17A antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant rat IL17-A protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:1,000 - 1:5,000, WB: 1:1,000
• Form/Appearance: Lyophilized
• Purity: This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant rat IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
• Conjugation: Unconjugated
• UniProt ID: Q61453
• NCBI: Q61453.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: None
• Alternative names: rabbit anti-IL-17A Antibody, rabbit anti-Interleuk
• Note: For research use only
• Application notes: This purified antibody has been tested for use in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µl by the LAL method. The recombinant immunogen is a non-glycosylated homodimer joined by disulfide bonds having a molecular mass of 30.0 kDa. By western blot from a reducing gel expect a band approximately 15.0 kDa in size corresponding to rat IL17-A protein in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Expression of atrial fibrillation (AF)-related pro-inflammatory cytokines at 4 days after surgery. (A) Fold mRNA expression of interleukin (IL)-6, IL-1β, transforming growth factor-β1 (TGF-β1) and IL-17A. (B) Protein expression of IL-17A detected by western blot analysis. (C) Quantitative analysis of IL-17A protein expression. *P< 0.05 and **P< 0.01 vs. Sham. #P< 0.05 and ##P< 0.01 vs. IgG2a. Sham, sham-operated rats; SP, rats with sterile pericarditis.

Western blot using Biorbyt's anti-IL-17A antibody shows detection of rat recombinant IL-17A protein (arrowhead, lane 1). Approximately 2 µg of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1000 dilution. The membrane was washed and reacted with a 1:20000 dilution of DyLight™ 649 conjugated Gt-a-Rabbit IgG. Expect ~15 kDa, or 30 kDa homodimer. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left. Other detection systems will yield similar results.

Western blot using Biorbyt's anti-IL-17A antibody shows detection of rat recombinant IL-17A protein (lane 1). Approximately 2 µg of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1000 dilution. The membrane was washed and reacted with a 1:20000 dilution of DyLight™ 649 conjugated Gt-a-Rabbit IgG. Expect ~15 kDa, or 30 kDa homodimer. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left. Other detection systems will yield similar results.