IL-17A antibody

• Catalog Number: orb345132
• Category: Antibodies
• Description: IL-17A antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: IL-17A Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL17-A protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:1:000 - 1:5,000, IHC: 1:100, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-IL-17A Antibody was affinity purified from monospecific antiserum by Protein A Purification. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended. Cross-reactivity with IL-17 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q16552
• NCBI: AAH662531.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Alternative names: rabbit anti-Interleukin-17A antibody, rabbit anti-
• Note: For research use only
• Application notes: IL-17-A antibody has been tested for use in IHC and western blotting and is suitable in ELISA. By western blot a band approximately ~17.5 kDa in size corresponding to human IL-17-A protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry of Rabbit Anti-human IL-17A Antibody. Tissue A: Human Small intestine H&E. Tissue B: IL-17A in Small intestine (20X). Tissue C: IL-17A in Small intestine (40X). Antigen Retrieval: HIER using Citrate Buffer for 20 min. Primary Antibody: anti-IL-17A at 1:100 at RT for 30 min. Secondary Antibody: Ready To Use Anti-Rabbit Poly-HRP IgG at RT for 8 min. Staining: DAB. Counter Stain: Hematoxylin.

Western blot using Biorbyt's anti-Human IL17-A antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG secondary antibody diluted 1:20000 in blocking buffer (p/n orb348637).