IL-17A antibody

• Catalog Number: orb345131
• Category: Antibodies
• Description: IL-17A antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: IL-17A Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL17-A protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:1,000 - 1:5,000, IHC: 1:100, WB: 1:500 - 1:2,000
• Form/Appearance: Lyophilized
• Purity: Anti-IL-17 Antibody was affinity purified from monospecific antiserum by Protein A Purification. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-17A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended. Cross-reactivity with IL-17 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q16552
• NCBI: AAH662531.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Interleukin-17A antibody, rabbit anti-
• Note: For research use only
• Application notes: Il-17A antibody has been tested for use in IHC and western blotting and is suitable in ELISA. By western blot a band approximately ~17.5 kDa in size corresponding to human IL17-A protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry of Rabbit Anti-human IL-17A Antibody. Tissue A: Human Small intestine H&E. Tissue B: IL-17A in Small intestine (20X). Tissue C: IL-17A in Small intestine (40X). Antigen Retrieval: HIER using Citrate Buffer for 20 min. Primary Antibody: anti-IL-17A at 1:100 at RT for 30 min. Secondary Antibody: Ready To Use Anti-Rabbit Poly-HRP IgG at RT for 8 min. Staining: DAB. Counter Stain: Hematoxylin.

Western blot using Biorbyt's anti-Human IL17-A antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG secondary antibody diluted 1:20000 in blocking buffer (p/n orb348637).