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Catalog Number | orb345106 |
---|---|
Category | Antibodies |
Description | IL-17A antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 4H1524.1 |
Tested applications | ELISA, FC, WB |
Reactivity | Human |
Isotype | IgG2b |
Immunogen | Anti-IL-17A (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17A produced in E.coli followed by hybridoma development. |
Concentration | 1.00 mg/ml |
Dilution range | ELISA: 1:10,000-1:50,000, FC: User Optimized, WB: 1µg/mL |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-Human IL-17A (MOUSE) Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17A protein. A BLAST analysis was used to suggest cross-reactivity with IL-17A from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17A from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | Q16552 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | mouse anti-Interleukin-17A antibody, mouse anti-IL Read more... |
Note | For research use only |
Application notes | Anti-Human IL-17A antibody has been tested for use in Western Blot and suitable in FLOW. Specific conditions for reactivity should be optimized by the end user. |
Expiration Date | 12 months from date of receipt. |
Western Blot of Mouse Anti-IL-17A antibody. Lane 1: human full length recombinant IL-17A protein. Lane 2: mouse full length recombinant IL-17A protein. Lane 3: rat full length recombinant IL-17A protein. Load: 20 ng/lane. Primary antibody: Anti-IL-17A antibody at 1 ug/ml for overnight at 4°C.
Western Blot results of Mouse Anti-IL-17A Antibody. Lane 1: Opal Prestained Molecular Weight Ladder. Lane 2: IL-17A. Load: 50 ng. Primary Antibody: Mouse Anti-IL-17A at 1 µg/mL overnight at 4°C. Secondary Antibody: Rabbit anti-Mouse HRP at 1:40000 for 30 min at RT. Blocking: BlockOut (p/n orb348644) for 30 min at RT.
ELISA, IF, IHC-P, WB | |
Bovine, Porcine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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