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Catalog Number | orb1231338 |
---|---|
Category | Proteins |
Description | IL-15 Recombinant Protein |
Tested applications | ELISA, WB |
Tag | Fc Tag |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by SDS-PAGE. |
MW | 39.7 kDa |
Target | Il15 |
UniProt ID | P48346 |
Protein Sequence | Asn 49 - Ser 162 |
Source | HEK293 cells |
NCBI | NP_032383.1 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
Alternative names | IL-15,Interleukin-15,MGC9721 Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 39.7 kDa. The protein migrates as 49 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Mouse IL-15, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Mouse IL-15, Fc Tag at 2 ug/ml (100 uL/well) can bind Biotinylated Human IL-15 R alpha, Fc, Avitag with a linear range of 10-156 ng/mL.
Greater than 95% as determined by SDS-PAGE. | |
12.5 kDa | |
E.coli |
> 97% as determined by SDS-PAGE and HPLC. | |
12.9 kDa | |
E.Coli |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 38.9 kDa after removal of the signal peptide. The apparent molecular mass of IL15-hFc is approximately 35-55kDa due to glycosylation. | |
Mammalian |
Greater than 95% as determined by reducing SDS-PAGE. | |
46.9 KDa | |
Mammalian |
Greater than 95% as determined by reducing SDS-PAGE. | |
12.5 KDa | |
Mammalian |