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Catalog Number | orb1231344 |
---|---|
Category | Proteins |
Description | IL-13 R alpha 2 Recombinant Protein |
Tested applications | ELISA, WB |
Tag | His Tag |
Form/Appearance | Lyophilized |
Purity | > 90% as determined by SDS-PAGE. |
MW | 39.0 kDa |
Target | IL13RA2 |
UniProt ID | Q14627 |
Protein Sequence | Asp 27 - Arg 343 |
Source | HEK293 cells |
NCBI | NP_000631.1 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.4 |
Alternative names | IL13RA2,CD213A2,CT19,IL-13R,IL13BP Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 39.0 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Human IL-13 R alpha 2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human IL-13, Fc Tag at 5 ug/ml (100 uL/well) can bind Human IL-13 R alpha 2, His Tag with a linear range of 4-31 ng/mL.
Immobilized Human IL-13 R alpha 2, His Tag at 1 ug/ml (100 uL/well) can bind Monoclonal Anti-IL-13 R alpha 2 Antibody, Mouse IgG1 with a linear range of 0.1-3 ng/mL.
> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC | |
63.4 kDa (predicted). Due to glycosylation, the protein migrates to 78-90 kDa based on Tris-Bis PAGE result. |
98.00% | |
39.5 kDa (predicted). Due to glycosylation, the protein migrates to 55-75 kDa based on Tris-Bis PAGE result. |
98.00% | |
63.7 kDa (predicted). Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result. |
> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC | |
37.55 kDa (predicted). Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result. |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Recombinant Human IL-13 R alpha 2 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Asp27-Leu342 ) of Human IL-13 R alpha 2 fused with hFc His Tag tag at the C-terminal. |