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Catalog Number | orb1230693 |
---|---|
Category | Proteins |
Description | IL-12 Recombinant Protein |
Form/Appearance | Lyophilized |
Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
Target | Il12a |
UniProt ID | P43431 |
Protein Sequence | p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS |
Source | CHO cells |
Biological Activity | Determined by its ability to induce cell proliferation in T-cell enriched PBMCs. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. |
NCBI | NP_032377.1 |
Storage | The lyophilized IL-12 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted IL-12 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing IL-12 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
Alternative names | p35, Ll12a, Il-12a, IL-12p35, Interleukin-12 subun Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
> 95% as determined by SDS-PAGE and HPLC. | |
57.2 kDa. 75.0 kDa is the observed value of non-reducing gel,and the reducing glue is the size of p40 and p35 subunits, and 57.2 kDa is the theoretical value. | |
Baculovirus |
ELISA, WB | |
> 90% as determined by SDS-PAGE. | |
59.0 kDa | |
HEK293 cells |
ELISA, WB | |
> 95% as determined by reduced SDS-PAGE. | |
83.5 kDa | |
HEK293 cells |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
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